WebHiTrap ® Desalting is a ready-to-use 5 mL column, prepacked with Sephadex ® G-25 Superfine, for fast and convenient desalting and buffer exchange. HiTrap ® Desalting … Web17-1408-01 HiTrap Desalting 5 × 5 ml 11-0003-29 HiTrap Desalting 100 × 5 ml* * Special pack delivered on specific customer order. Connectorkit Connectors supplied Usage No. supplied 1/16” male/luer female Connection of syringe to top of HiTrap column 1 Tubing connector Connection of tubing (e.g. Peristaltic flangeless/M6 female Pump P1) to ...

HiTrap Desalting columns with Sephadex G-25 resin - Cytiva

WebDesalting and buffer exchange are methods to separate soluble macromolecules from smaller molecules (desalting) or replace the buffer system used for another one suitable for a downstream application (buffer exchange). These methods are based on gel filtration chromatography, also called molecular sieve chromatography, which is a form of size … WebThe effect of different sample volumes on the HiTrap Desalting column using tubing loops for sample injection. Column: HiTrap Desalting Sample: 2 mg/ml bovine serum albumin … pipe heat cord

Dialysis and Desalting Fisher Scientific

WebThe effect of different sample volumes on the HiTrap Desalting column using tubing loops for sample injection. Column: HiTrap Desalting Sample: 2 mg/ml bovine serum albumin in 50 mM sodium phosphate buffer, 0.5 M sodium chloride, pH 7.0 Sample volume: 0.5 ml, 1.1 ml, 2.1 ml Eluent: 50 mM sodium phosphate buffer, 0.15 M sodium chloride, pH 7.0 WebThe choice of HiTrap column size depends on the amount of expressed protein. While in this example a HiTrap Chelating HP 1 ml column is used, a HiTrap Chelating HP 5 ml is also available and should be used if the expected amount of recombinant protein exceeds 10 mg. For further scaling-up, Chelating Sepharose™ Fast Flow is available. 1.0 0.75 ... WebHiPrep Desalting(26ml)可在数分钟为多至10ml样品脱盐。 选择孔径很小的凝胶，在过滤时，由于蛋白质分子半径较大，所以不能进入凝胶的孔隙，很容易被洗脱，而粒子则被孔 … pipe heater jacket